IVF-ET is needed to be done is patients with blocked fallopian tubes. It may also be done in other forms of infertility where IUI super ovulation has failed. In this the woman is subjected to controlled ovarian hyperstimulation using hormonal injections. Many more injection are required because we want to retrieve as many eggs as possible. Once the follicles have reached an appropriate size, vaginal ovum aspiration is done and the ova are collected in a petridish with a media. Capacitated sperms are then mixed with oocytes and fertilisation is achieved into the uterus. Once embryos are formed then they (2-3 embryos) are transferred into the uteros on day 3 or day 5. Progesterone support is then given chemical pregnancy is diagnosed by B-HCG on day 30. Live pregnancy is confirmed by 5 weeks by seeing a live foetal heart on vaginal sonography.

This is mainly done for blocked tubes, unexplained infertility, PCO, Endometriosis grade 3-4, mild oligospermia in males. Hormonal injections given to woman to induce super ovulation, serial Transvaginal sonographies done to monitor follicles, HCG injections given to induce release of eggs, Ovum pick up done with trans vaginal sonography, eggs are inseminated with processed sperms of her husband. Fertilization of eggs takes place within a day followed by cleavage & then embryos thus formed are replaced into uterus on day 2, 3 or 5 (Blastocyst). Patient is given Luteal phase support with injectable vaginal progesterone pessaries.
IVF is a four-stage procedure:
Stage One: Hormonal injections are given to stimulate the development of multiple follicles.
Stage Two:Once mature, the eggs are removed (or retrieved) from the woman’s ovaries using a fine needle.
Stage Three:The eggs are transferred to a laboratory dish where they are fertilized by sperm collected from the male partner.
Stage Four:Several days later, the fertilized embryo is inserted back into the uterus.

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